 This study investigated the effectiveness of polyvinyl pyrrolidone-coated silver nanoparticles, PVP-coated AGNPs, as a potential topical vaginal microbicide to prevent transmission of HIV-1 infection. The results showed that PVP-coated AGNPs were effective in blocking the transmission of both cell-associated HIV-1 and cell-free HIV-1 isolates. Additionally, only one minute of exposure to PVP-coated AGNPs was sufficient to protect the cervical tissue from infection. Furthermore, the PVP-coated AGNPs did not cause any cytotoxicity to the cervical tissue. Therefore, PVP-coated AGNPs may be a promising microbicidal candidate for use in topical vaginal cervical agents to prevent HIV-1 transmission. This article was authored by Rodriguez Padilla-Cristina, Garza Trevino-Elsa N., Xtopantarant Liliana, and others.