 Okay everybody, Dr. O here. Quick video, I just want to walk you through the bacterial streak plate method that I have you use in the laboratory or at least the one that I would want you to try to use the first time you tried to make a streak plate. So streak plates and pore plates are the two methods that we use in our laboratory to spread out bacteria so we can isolate individual pure colonies. So I won't go through all of the details here but mainly just the technique on the plate itself but you should obviously sterilize your loop until the entire wire portion is red hot, let it cool and then use your aseptic laboratory techniques when you get your microorganisms. But now we're ready to streak on the plates. We're gonna open the lid, how far are we gonna open it, you know just far enough to minimize air contamination and how I like you to do it the first time is to reach across the plate and then originally do five streaks. So one, two, three, four, five streaks with your wire loop. The best tips I can give you here is to make sure you come in nice and low so you're using the flat circular portion of the loop and not gouging the plate with the end and then also make sure that these these streaks are as long as possible so from edge to edge on the circle because remember we're trying to rub bacteria off so that we get individual bacteria creating isolated colonies somewhere here in the middle of this plate. So one, two, three, four, five streaks. Then what I would have you do is sterilize the loop. You want to kill all the remaining organisms and remove all of them that are still on the loop. That way the only bacteria you're working with are from these five streaks that you left on the plate. So sterilize your loop, you've closed your plate, turn it 90 degrees and then now you're going to do one, two, three, four streaks. And you'll notice here in the animation that I'm adding into this later that those four streaks have to cross the original five streaks. So where are the bacteria coming from? The only bacteria that you're going to be working with on this plate now, we're a portion of those first five streaks. So then I do not have you sterilized again. I've generally found that that one sterilization step is enough. If you're making plates and there still aren't isolated colonies, there's still too many bacteria, you could certainly try sterilizing again, but I generally don't recommend it or see a need to do it. So you've done your first five lines, you've sterilized, you've done your second four lines. Now you're going to turn it 90 degrees again and you're going to do three lines. One, two, three in the direction I'm showing you here. And then you're going to close it, turn it another 90 degrees and then just do one last streak through those three lines and then just swirl right through the middle of the plate until you've used the entire surface. So the biggest mistakes that I see when making streak plates are number one, not using the whole plate. So make sure your lines are about as long as they can be. So you can really spread the bacteria thin. Number two, your lines need to cross the lines before them. That's where you actually will draw and pull the bacteria into these new lines. So that's very important as well. So, okay, I hope this helps. I hope this technique works really well for you. We'll cover the poor plate method that we use, what are called serial dilutions in a different video. All right, have a wonderful day. Be blessed.