0:40 How does CRISPR-Cas9 work?
2:35 A standard experiment
4:35 Challenges in genome editing
5:03 Choosing the right approach (plasmid, mRNA, protein)
6:35 Plasmid DNA
8:13 RNA - case study
11:52 RNP - case study
15:20 Is cas9 the only one?
16:18 Tips & tricks for genome editing optimization
19:45 CRISPR Toolbox (Polyplus products)
20:15 in-vivo genome editing
Check out this new webinar made by our experts in transfection on CRISPR-Cas9 genome editing system.
When using CRISPR/Cas9 system, transfection is a key and often limiting step to ensure successful genome editing. There are three main systems to transfect guide RNA and express Cas9 protein which are DNA, RNA or RNP (gRNA + Cas9 protein) approaches. Each system has their own pros and cons in terms of easiness of use, genome editing efficiency and off-targeting effects.
Learn more about it on our website: https://www.polyplus-transf...