Fijiwingsadministrator

Here we present FijiWings, a set of macros designed to perform semi-automated morphophometric analysis of a wing photomicrograph. FijiWings uses plug-ins installed in the Fiji version of ImageJ to rapidly and accurately measure wing area, reliably detect trichome positions and calculate trichome density of a wing region selected by the user. For more high-throughput screens we have developed a macro that directs a trainable segmentation plug-in to detect wing vein locations and use this information to both measure trichome density in specific intervein regions and generate an overall 'heat map' of relative intervein areas. At "https://sourceforge.net/projects/fijiwings/" click on "Files" in the menu to download Fijiwings for Mac and Windows and find installation instructions there on "Readme.txt."