ORGANIC REACTIONS IN LIVE CELLS: A new fluorogenic click reaction for selective cell-labeling
Fluorescent probes are an indispensable tool in biological research. They enable the study of biological processes and the specific biomolecules involved in them. Particularly important are probes that become fluorescent only when they react with the biological target of interest. However, the development of such tools is difficult and complex chemical synthesis is often needed for their preparation.
Dr. Milan Vrábel and his team from the Institute of Organic Chemistry and Biochemistry in Prague (IOCB Prague) have now developed a novel chemical reaction, which significantly simplifies the whole process.
One example of this powerful methodology allows for the imaging the place of action of anti-cancer drugs in live cancer cells almost in real time.
Vázquez, A., Dzijak, R., Dračínský, M., Rampmaier, R., Siegl, S. J. and Vrabel, M. Mechanism-Based Fluorogenic trans-Cyclooctene–Tetrazine Cycloaddition. Angew. Chem. Int. Ed. 2017, 56, 1334–1337. doi:10.1002/anie.201610491