NGS101 — Dr. Lei Guo

Step-by-step NGS data analysis tutorials for wet-lab biologists, medical doctors, and researchers — no programming experience required.

I'm Dr. Lei Guo, a Computational Biologist at UT Southwestern Medical Center and founder of NGS101.com, where I've published 70+ in-depth tutorials covering RNA-seq, single-cell RNA-seq, ChIP-seq, ATAC-seq, WGS/WES, Hi-C, DNA methylation, and more.

Having collaborated with hundreds of bench scientists and clinicians, I understand exactly where beginners get stuck — and I teach the way I wish someone had taught me: clearly, step by step, with zero assumed knowledge.

🔬 What you'll find here:
- Complete beginner series: RNA-seq, WGS, scRNA-seq, ChIP-seq, ATAC-seq, Hi-C, DNA methylation
- Every tool explained from scratch — STAR, GATK, DESeq2, Seurat, and more
- Code you can use on your own data immediately

📖 70+ written tutorials → ngs101.com