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Neuroslicer uploaded a new video
(8 months ago)
David Hubel and Torsten Wiesel recorded from neurons in the visual cortex of cats and mapped out their receptive fields. They found many neurons w...
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David Hubel and Torsten Wiesel recorded from neurons in the visual cortex of cats and mapped out their receptive fields. They found many neurons were excited by particular patterns of light or darkness, patterns such as dots, circles and bars. They also found neurons that were sensitive to shape orientation and movement. In this video they project these patterns of light onto a screen placed in front of the animal while recording from neurons in the visual cortex. The popping clicking sounds you hear correspond to electrical activity from these recorded nerve cells played over an audio monitor.
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Neuroslicer uploaded a new video
(8 months ago)
This is a small dendritic branch from a Layer 3 neuron in the mouse auditory cortex. The neuron has been filled with a red fluorescent dye (Alexa ...
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This is a small dendritic branch from a Layer 3 neuron in the mouse auditory cortex. The neuron has been filled with a red fluorescent dye (Alexa 594), which is why it appears red on the video. It has also been filled with a calcium sensitive green fluorescent dye (Fluo 5F). When neurons from the thalamus are stimulated (during the yellow dot on the video), you will see that one of the dendritic spines (shown by the arrow) glows green, showing that it is being activated by thalamic stimulation. Using this mapping technique, we now know that thalamic neurons synapse very close to the cell body of these auditory neurons, helping to guarantee that they reliably receive sound information from the environment.
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Neuroslicer uploaded a new video
(8 months ago)
This purkinje neuron from a mouse cerebellum was imaged with the fluorescent dye Alexa 594, injected into the cell with a microelectrode. The high...
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This purkinje neuron from a mouse cerebellum was imaged with the fluorescent dye Alexa 594, injected into the cell with a microelectrode. The high resolution image was captured with a two photon laser scanning microscope. The microscope took two dimensional images of the neuron, and then advanced deeper into the tissue, one micron at a time, taking many more scans. The computer took the stack of images and reconstructed the 3D video you see here.
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