 This paper presents a novel application of the 454-GSFLX platform, which allows the genotyping of thousands of samples in one run. A probabilistic model was developed to demonstrate the reliability of this method, and it was successfully applied to the genotyping of 1,407 samples from 24 different rodent species. The authors estimate that the reproducibility of genotyping reached 95%, suggesting that this method could be used to accurately identify individual variations in genetic markers. This new approach offers great potential for the study of evolutionary and functional genetics of highly polymorphic genes such as MHC genes in vertebrates or locky-regulating self-compatibility in plants. Additionally, this method can be used to detect individual variation in disease susceptibility, as well as in productivity or disease susceptibility traits in agriculture. This article was authored by Charbonneau-Natalie, Courageil, Givia Emmanuel, and others. We are article.tv, links in the description below.