 This study investigated the effect of three genetic variants RS7482144, RS766432, and RS9399137 on hemoglobin F, HBF levels in patients with allocemia major and intermediate. The results showed that the HBF levels were significantly correlated with the presence of the variant allele of RS7482144 in the HBG2 gene, as well as with the variant allele of RS766432 in the BCL11A gene. Furthermore, the presence of the variant allele of RS9399137 in the HBS1L MYB gene did not have any significant impact on HBF levels. Overall, this study suggests that the presence of certain genetic variants can affect HBF levels in patients with allocemia. This article was authored by Shabnam Bashir, Sakib Mahmud, Shahida Mohsen, and others.