 Dear students, my name is Mohamed Raschar and today we are going to perform an experiment named as isolation of Lactobacillus from dairy product. In order to perform this experiment, the material, apparatus and chemical which we will use, let me introduce with that practice. We need pipettes, goat milk, glass test tubes, test tube rack, L shape spreader, blue tips, prepared MRS agar plates and distal water. So in order to perform this experiment, we need aseptic conditions but that we will perform this experiment in laminar air flow so that we can maintain aseptic condition. So let's begin with the experiment. So in the very first step of this experiment, we will perform the serial dilution of goat milk. In order to perform the serial dilution of goat milk, we will take a micropepper and take 1 ml of goat milk and mix it into 9 ml of distal water. And from second tube in which we have just added 1 ml of goat milk, we will take 1 ml of solution and we will add into the third tube and likewise we will transfer it up to tube number 7. After making the serial dilution, we will take 1 ml of solution from the last test tube and will spread over the already prepared MRS agar plates. Once we will pour 1 ml of solution on already prepared MRS plates with the help of L shape spreader, we will spread that solution. After spreading that solution, we will simply incubate these plates at 37 degree centigrade for 24 hours in order to take the growth of lactobacillus species. So let's begin with this experiment. Get these plates at 37 degree centigrade for 24 hours and after passing 24 hours, we will observe the growth. So students, after 24 hours incubation, you can clearly observe the growth of lactobacillus in Petri plates.