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Published on Nov 19, 2013
in vitro time-lapse movie of neural crest cells expressing EB3-GFP to allow visualisation of growing microtubules. Right panel shows the same sequence with overlapping consecutive frames to make microtubule trajectories more evident
In a recent Development paper, Roberto Mayor and colleagues studied the role of the polarity protein Par3 in contact inhibition of locomotion (CIL) during the migration of neural crest cells. By labelling neural crest cells with EB3-GFP, which allows the visualisation of growing microtubules, the authors were able to show that wild type neural crest cells experience a greater proportion of microtubules catastrophe at contact points than at free edges. When Par3 is mutated, however, this increase in catastrophe at contact points is abolished. This result suggests that Par3 is required at cell-cell contacts to promote microtubule catastrophe.