Rating is available when the video has been rented.
This feature is not available right now. Please try again later.
Published on Mar 11, 2014
For more info, visit http://www.bio-rad.com/yt/3/2-D_Elect... This 45 minute webinar is for 2-D novices and experts alike who want to hone their skills and keep abreast of new advances in the field. Dr. Anton Posch, a renowned expert in 1- and 2-D electrophoresis, provides insights on sample preparation, isoelectric focusing (IEF), SDS-PAGE, and image analysis for 2-D electrophoresis. Included are techniques to improve reproducibility and minimize experimental error. He also shows how to troubleshoot issues with your 2-D protocol as well as protein fractionation and western blotting image analysis.
Part II covers IEF separation, using immobilized pH gradient (IPG) strips, running second-dimension PAGE, spot detection, gel matching, and data analysis, followed by Q&A on topics including sample preparation, storage, and running conditions. Also see 2-D Electrophoresis Tips & Tricks - Part I for the main steps of the 2DE workflow, starting with sample preparation including extraction and solubilization, post-extraction protein protection, and critical success factors for good yields of intact proteins at detectable levels.
Dr. Posch has provided hands-on training in this field for 19 years and has more than 30 peer-reviewed publications. He currently sits on the editorial boards of both Proteomics and Proteomics -- Clinical Applications and is the editor of "2-D PAGE: Sample Preparation and Fractionation," among the top 25% books downloaded in the 2012 Springer eBook collection.
Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges.