 My research is about investigations of risks associated with tropical bacterial pathogens under various level 3 conditions. I have been working at Mahidol and Oxford Tropical Medicine Research Unit since 2009 As bio-safety officer, the last two decades of gam-negative microorganisms predominant in laboratory and hospital acquire infections. This database can help bio-safety officer to carry out quantitative list assessment of laboratory procedures. I have three experiments of my study. First one, I need to determine the number of bacteria that can survive on many types of material that we use to build the laboratory facility. For the second experiment, we try to count the number and investigate the efficiency of disinfectant that can decontaminate the bacteria that lab workers use to decontaminate equipment in the laboratory. And the last one, we decide a series of accident and we will count the number of bacteria in each activities by using sampling machines. I have joined some of our lightening course with the Open University so that course is useful for me in part of my project. Some microorganisms can cause human disease. I hope this database can help people who are working in the laboratory to work with microorganisms safely.