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How to Count Molecules on SlipChip Using a Cell Phone

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Published on Nov 12, 2013

This video demonstrates that a minimally trained user can implement a step in the quantitative molecular diagnostic approach described in the following paper:

David A. Selck, Mikhail A. Karymov, Bing Sun, and Rustem F. Ismagilov, "Increased Robustness of Single-Molecule Counting with Microfluidics, Digital Isothermal Amplification, and a Mobile Phone versus Real-Time Kinetic Measurements," Analytical Chemistry 2013, doi: 10.1021/ac4030413.

After single molecules were amplified on SlipChip, a cell phone was used to image the chip in a shoebox. The image was then sent to a remote server, where the pattern of "positive" and "negative" wells on the chip was automatically analyzed, Poisson statistics was applied, and the number of molecules present in the sample was calculated. Quantitative results were automatically sent via email. The sample in the video contained strands of RNA extracted from a non-infectious virus, posing no potential danger to the child demonstrating the technique.

To see how a SlipChip operates, see: https://www.youtube.com/watch?v=gyc57...

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