 A diet questionnaire, DQ, designed to assess habitual diet and phytoestrogen intake was developed. The DQ was validated against alkyl resorcional metabolites measured in urine as objective biomarkers of whole grain wheat and rye intake. We found a statistically significant correlation between the DQ and FR for reported whole grain intake and isoflavonoids, as well as for intake of macronutrients. This article was authored by Sana Nibaka, Helen Berthouse-Forcelund and Maria Hedeline.