 The MTT antibacterial assay is a quick and efficient way to measure the antibacterial activity of natural compounds. However, it can be difficult to accurately measure the results due to the presence of background color and bacterial cells. To overcome these issues, a silica gel column chromatography method was developed which combines with the MTT assay to remove the background color and bacterial cells prior to the formation measurement stage. This method is particularly useful when testing phytochemical extracts that have an interfering color. This article was authored by Don Shasher when Leon Su, Marie Wong, and others.