 This study compared the performance of two commonly used yeast species, Pitchia pastoris and Hansenula polymorpha, when producing the L1 protein of the human papilloma virus, HPV. Both yeasts were able to produce the L1 protein at high levels, with P. pastoris showing slightly better results than H. Polymorpha. Additionally, the study used reverse vaccinology to design alternative multi-epitope vaccines in recombinant protein and M. INA forms. The resulting vaccines were shown to activate the immune system and are predicted to be safe. This study provides valuable information about the potential use of these yeasts for large-scale production of HPV-52 vaccines. This article was offered by Moe G. Raman Ferdorz, A. Ponzino-Mastopa, Nalei Ekewati, and others.