 This study investigates the effect of binding events on the work function of gold surfaces. It was found that binding events can cause a significant decrease in the work function of gold surfaces, which can be detected using Kelvin probe force microscopy. Additionally, it was observed that binding events do not affect the work function of gold surfaces when they are exposed to non-binding antibody solutions. This article was authored by Cynthia D. Franco, Eleonora Machia, Lucia Sarsina, and others.