 Cancer cells use lipid metabolism to produce energy and build up biomolecules for rapid growth. Fluorescence microscopy is often used for cancer research, but it cannot label small lipid molecules without disturbing their natural activity. However, coherent Roman scattering, CRS, microscopy can overcome this challenge by providing chemical selectivity, high sensitivity, sub-micrometer resolution, and fast imaging of lipid molecules in live cells. Recently developed hyperspectral and multiplex CRS microscopy further enhances molecular selectivity by using distinct Roman peaks to identify different lipid metabolites. These advancements have enabled researchers to gain new insights into the role of lipid metabolism in cancer development and progression. This article was authored by Shuo Zhang, Ye Shuan He, and Xu Huayu. We are article.tv, links in the description below.