 In this study, researchers developed a new hydrogel system consisting of sodium alginate, SA, basic chitosan, CS, and silver nanoparticles, AGNPs. The SA AGNPs were prepared using either ascorbic acid or microwave heating and then added to the CS solution. The resulting hydrogel was tested for its antimicrobial properties against Escherichia coli and bacillus subtilis. The results showed that the SA AGNPs slash CS hydrogel had a significantly higher antibacterial effect compared to the SA slash CS hydrogel. Furthermore, the SA AGNPs slash CS hydrogel also demonstrated improved mechanical strength and lower cytotoxicity compared to the SA slash CS hydrogel. This article was authored by Takuma Owee, Duankumal Decojerasri, Sachiro Kokonoki, and others.