 The study examined the effects of overexpressing four genes involved in the synthesis of isobutinol in Sacramuses cerevisiae. The results indicated that overexpression of these genes resulted in an increase in isobutinol production in S. cerevisiae. Additionally, the study found that providing amino acids in the culture medium improved isobutinol production in the reference strain, but did not improve isobutinol production in the ILV-2, ILV-3, ILV-5 overexpression strain. This suggests that other factors beyond the activity of the genes are limiting isobutinol production in this strain. This article was authored by Kahumal Kaisa, Keeland Brant Morton C., Borodino Arena, and others.