 Hi, I'm Brigid Karaher. I'm co-director of the Simon's Electron Microscopy Center, the New York Structural Biology Center and a whole center of centers. And I'm also currently the founding technical director of the Chan Zuckerberg Imaging Institute. So what are the benefits of using CryoM technology? There are so many, I think, at this stage because CryoM is many different pipelines at the moment. There's a single particle cryoM pipeline and the benefit there is mostly that you can get these high resolution structure of beautiful proteins and protein complexes, things that are intractable to other methods like X-ray crystallography and NMR. But then there's another whole pipeline we call CryoElectron Tomography and really what that lets us do is look inside cells and see what proteins and protein complexes are doing inside cells, which may be different than what they look like in vitro. So there are many, many benefits of CryoElectron Microscopy and CryoElectron Tomography, basically looking at proteins, protein complexes in situ at very high resolution. There's many bottlenecks in the pipeline. I think the single particle CryoElectron Microscopy, the main bottleneck right now is in making the specimen in the form that we needed in the electron microscope, which has to be in a very thin form and as a result the protein sometimes interacts with the air water interface and that causes us all kinds of problems. For the CryoElectron Tomography pipeline there are many bottlenecks all the way along, making the specimen in the first place, getting enough contrast in the very crowded environment of the cell to actually find your structural interest, the particular molecule that you're looking for and then doing that processing to take months and months and months are very hard work with a lot of compute power and every single step along that way is difficult and has challenges and also there's a lot of opportunities for new technology development, which I'm sure will be coming. So making this technology more accessible to the entire sign to the community requires I think a lot of automation, a lot of streamlining and this would be a lot less cost because it's a very, very expensive technology both to acquire the hardware in the first place, to look after that hardware, to maintain it and then to run it, you need experts to run it. So a lot of automation and streamlining and cost reduction could help. The other thing that helps it somewhat I think is to have very large centers. We run a large center, a couple of large centers in New York City and there are many others around the world, Technopole being one of them and you know making those sort of centralized facilities where you where there's an economy of scale is very important I think to democratizing these methods. So I think Technopole is doing everything right. I'm very, very impressed by what you've done here. I think you have a beautiful array of equipment and it's very complete. That was quite impressive how complete the equipment is and you're doing training, bringing people from the outside, having beautifully well supported platforms. All of that is exactly spot on right and I think you've done a beautiful job in going in exactly the right direction.