 The optimal enzymatic cocktail for hydrolyzing AFEX-pre-treated corn stover consists of a combination of two xylinases, LX3 and LX4, and three cellulases, Cbhi, Cbh2 and Egi, at a mass ratio of 25 to 75. Additionally, the addition of 0.6 mg per G-glucon of xylosidase, Lx, and 0.6 mg per G-glucon of arabinofuranosidase, L-arb, increases the monomeric xylos yield by 20%, while the addition of 0.8 mg per G-glucon of glucuronidase, Lgl, further increases the xylos yield by another 20%. Furthermore, this cocktail has been shown to reduce the total enzyme loading required for equivalent yields when compared to Accelerace 1000, a commercially available enzyme cocktail. This article was authored by Hermansson-Spencer, Ushering, Chunderwatcheshi PS, and others.