 Thank you for rising and shining after what turned into a fairly intense but interesting and long day yesterday. So this morning we're going to kick off the session with topic two, using genomic assays of function to interpret the role of genetic variation in disease. I wanted to kick off this morning with just reminding people of the discussion questions that hopefully will guide some comments by the speakers and should guide the discussion after each speaker about types of projects, systems, obviously for this session the system is the human, the live human, the utility of generating new data versus exploiting and not versus and exploiting new methods, infrastructure needs, organizational structures, what opportunities exist for synergies and the appropriate scale. And I think if we can come back to these questions periodically, not let them drive us, but come back to them periodically, it will benefit NHGRI leadership. So our first speaker today, this morning is Ervin to Chakravarti. We're going to reverse the order of the first two speakers from Johns Hopkins University. Ervin is going to talk about Hirschsprung's disease consequent to mutations in the RET gene regulatory network.