 In this study, it was found that 1.5 percent of patients registered in the tuberculosis program in Cali. Columbia had nontuberculosis mycobacteria isolation instead of mycobacterium tuberculosis. This suggests that nontuberculosis mycobacterial infections are more prevalent than previously thought in this population. Additionally, 86 percent of these patients received unnecessary tuberculosis treatment due to the lack of clinical suspicion and the limited laboratory infrastructure. Therefore, it is recommended that nontuberculosis mycobacteria identification should be considered for public health interventions. This article was authored by Luis Eduardo del Daniela Roccio Escobar, Diana Marcella-Oyos, and others.