 Co-FE204 nanoparticles were prepared by the co-precipitation method and then surface modified with capsaicin, capsicum-annuum SSP, for antimicrobial and photocatalytic activities. The virgin Co-FE204NPs and capsaicin-coated Co-FE204NPs, CPCFNPs, were characterized by XRD, FDIR, SEM, and TEM. The antimicrobial potential and photocatalytic degradation efficiencies of the prepared samples via FUXIN BASIC, FB, were investigated. The results revealed that Co-FE204NPs have spherical shapes and their diameter varied from 18.0 to 30.0 nanometers with an average particle size of 25.0 nanometers. Antimicrobial activity was tested on gram-positive staphylococcus aureus ATCC 52923 and gram-negative ischicoli ATCC 52922 strains. The zone of inhibition, ZOI, and minimum inhibitory.