 The present work involves the dyeing of cotton fabrics with natural dye extracted from calliganum comisum plant, followed by mordanting with four different mordants. The effects of mordants were analysed using XRD, FTIR, SEM, DSC and TGA techniques. The results showed that the ferrous chloride and ferrous sulfate mordants improved thermal stability and altered morphological structure of cotton fabrics, imparting antioxidant and antibacterial properties.