 The research aimed to determine the influence of amino acids and ascophilum nodosum filtrate on two broccoli cultivars' response to soil drought and found that application of biostimulants enhanced tolerance to drought stress, with agacy being more sensitive. Chlorophyll fluorescence measurements showed changes in photochemical efficiency, quantum yield, non-photochemical fluorescence quenching and photosynthetic electron transport rate, while chlorophyll content index was affected by the cultivar and biostimulant application. This article was authored by Alina Kaliswix, Renata Baxequinta, Vladimir Zasinski, and others.