 CRISPR-slash-Cas9 technology has the potential to significantly enhance plant breeding. However, it is important to consider the possibility of off-target mutations when designing SGRNAs. Our study suggests that off-target mutations can occur at positions with one or two mismatches, but are less likely to occur at positions with three or four mismatches. Furthermore, off-target mutations can also occur at positions with insertions or deletions relative to the SGRNA sequence. Therefore, it is important to take these factors into consideration when designing SGRNAs. This article was authored by Ellen Slaman, Ellen Slaman, Mikhail Lambers, and others.