 The study found that continuous aeration of slurries of pre-treated softwood supported the catalytic action of lytic polysaccharide monoxygenase, LPMO, and improved glucon conversion, while increasing substrate content decreased glucon conversion. Aerated reactions resulted in higher glucon conversion than N2, supplied reactions at all WIS values, with the largest improvements achieved at 17.5 percent WIS double enzyme dosage also improved glucon conversion, but to a lesser extent than aeration. Structural analyses revealed that aeration increased surface area, disorder, and diabsorption based on cellulose content while decreasing crystallinity. These results demonstrate the potential of using proper aeration to exploit LPMO's capacity in enzymatic saccharification of lignocellulosic substrates and provide detailed information about the characteristics of the substrate after interaction with cellulolytic enzymes. This article was authored by Chao Jun-Tang, Modavilla Togandla, and Leif J. Johnson. We are article.tv, links in the description below.