 The study aimed to evaluate the effect of copper sulfate, CUSO4, on the regeneration and salimerin production of syllable merianamal, milk thisyl, extracts. The results showed that increasing the CUSO4 concentration up to 5M and elicitation period up to seven days significantly enhanced the salimerin content, with a maximum value of 11.79 and 11.67 milligrams This article was authored by M. E. L. Charneby and FAMIAS Hassan.