 The comparison of three different next-generation sequencing platforms revealed that the Ion-Torrent PGM had the best overall performance, followed closely by the Illumina MISEC. The Pacific Biosciences RS showed some issues with its ability to accurately sequence highly AT-rich genomes, such as the Plasmodium falciparum genome. The Ion-Torrent PGM also performed better than the Pacific Biosciences RS when it came to variant calling, although the latter still managed to detect some variants. This article was offered by Quail Michael A., Smith Miriam, Copeland Paul, and others.