 A new HPLC method was developed for the simultaneous analysis of three antibiotics, erythromycin, azithromycin, and spiromycin using ODB-RP18 as a stationary phase. The method was optimized for use with a setinatrial 2-methyl-2-propanol hydrogen phosphate buffer, pH 6.5, with 1.5% triethylamine. It was found to be highly efficient with good selectivity and low levels of interference from other compounds. The method was also shown to have excellent accuracy and precision, with recoveries ranging from 97.13% to 100.28%. This method can therefore be used for the analysis of these antibiotics in pharmaceutical dosage forms such as tablets and powders for oral suspensions. This article was authored by Ammar Moudi, Sylvia de Francia, Mohamed Salabu Kechem, and others.