 The research aimed to evaluate the use of nanosilver particles for plant material disinfection, estimate a medium penicidum alopecuroids, and assess the influence of growth regulators on regeneration in vitro. The results showed that nanosilver particles reduced contamination levels and that the best results were obtained, when used at concentrations of 100-250 mgdm, 3 alone or as a supplementation of the media, at concentrations of 4 mgdm, 3 for nodes and 16 mgdm, 3 for adventitious buds. Nodal-explants resulted in less contamination, and the most regenerated shoots were obtained on MS media supplemented with 1 mgdm, 3 ba and 0.1 mgdm, 3 iba. This article was authored by Marzena Parsimis, Christina Pudelska, and Monica Ponywasik.