 This research study investigates the use of annato colorants derived from bixa or elona l-seeds for textile dyeing, specifically pre-mordanting wool with biantrimetal salt combinations to develop natural and beautiful shades on wool with variation in hue and tone. The dyeing was carried by exhaustion method and dyed samples were analyzed using a reflective spectrophotometer in terms of C-Lab, L, and B, and KS values and washing and light fastnesses were investigated according to i.s.o standard recommendations. The results showed that overall 36 different shades having good to very good fastness properties were produced by the use of different metallic salt combinations in the natural dyeing of wool using annato dye.