 Welcome back to MedSmarter where we're taking a smarter approach to preparing future physicians so let's continue on and say we have a Organism that is catalase negative, so it does not break down hydrogen peroxide That makes it a streptococcus. We can then grow these organisms on a blood auger plate And this will help us determine if they are alpha Hemolysis beta homolysis or gamma homolysis what we'll see without the homolysis is Basically a partial hemolysis is occurring and the blood auger plate will turn green If the blood auger plate undergoes complete hemolysis This is beta homolysis and you will see clear spaces on the blood auger plate And then if there are no hemolysis that occurs then you will see very little to no change in the blood I've got blood auger plate because it is a gamma homolysis where there is no homolysis occurring So we can now move forward and determine our streptococcus based upon this homolysis So if we have partial homolysis because the blood auger plate has turned green in the presence of that micro bacteria It is going to be one of these bottom two either strep mutans strep midis or strep pneumonia We need to determine which one of these we're dealing with so in that case we will check its sensitivity to Opticin as well as biosolubility If it is sensitive to optichin then it is strep pneumonia If it is not sensitive to optichin then we're talking about strep mutans and strep Midis If we have clear Presentation on the blood auger plate we have our beta hemolysis as we've discussed and we can then run those through a test checking it for sensitivity to basatration as well as the PYR status and the PYR status is the Pyrrolidinol test where we are determining if L Pyrrolidinol beta naphthalamide is hydrolyzed by a certain bacterial aminopeptidase enzymes so if we have basatration sensitivity and our PYR status is Positive then we are dealing with strep pyogenes if it is negative. We're dealing with strep agalactae and finally our gamma hemolysis if it grows under a 6.5% sodium chloride solution then we're dealing with enterococcus specifically enterococcus fecium and fecalis or If it does not grow under that 6.5 percent sodium chloride and pyro PYR status Then we are dealing with a non-entroococcus such as strep bovis Come back soon for some more discussions and breaking down each one of these organisms in more detail