 This research paper discusses the development of a multi-layered nanofibrous wound patch loaded with three drugs, phenytoin, sildenophilcitrate, and symbostatin, to treat diabetic ulcer wounds. The polylactic acid nanofiber matrix was prepared and incorporated with each drug in a separate layer. The drugs were released at different rates over a period of three weeks. Physicochemical and mechanical properties of the patches were assessed, along with their biodegradability, swellability, breathability, and porosity. Additionally, the patches were evaluated for their biocompatibility and cell adhesion capability. Finally, the patches were tested on rats with diabetic ulcers, and it was found that they successfully induced proper wound healing within three weeks. Histopathology revealed complete healing with proper cell regeneration and arrangement without forming scars. This article was authored by ISRA HLE, ISLAME KALIL, and Ibrahim ML SHERBANI. We are article.tv, links in the description below.