 1. Immunoinformatics tools were used to predict human leukocyte antigen, HLA, class I-restricted T-cell epitopes within the envelope glycoproteins and nucleocapsid proteins of Ebola virus, EBAV, and Sudan virus, SUDV, and the structural proteins of Venezuelan equine encephalitis virus, VEV. 2. Selected epitopes were tested for binding to soluble HLA molecules representing five class II alleles, DRB10101, DRB10301, DRB10401, DRB10701, and DRB11501. 3. All but one of the 25 tested peptids bound to at least one of the DRB1 alleles, and four of the peptids bound at least moderately or weakly to all five DRB1 alleles. 4. Additional algorithms were used to design a single string of beads expression construct with 40 for selected epitopes arranged to avoid creation of spurious junctional epitopes. 5. Seventeen of these 44 predicted epitopes were conserved between the major histocompatty complex, MHC, of humans and mice, allowing initial testing in mice. 6. BALB-C mice vaccinated with the multi-epitope construct developed statistically significant cellular immune responses to EBAV, SUDV, and VEV peptids as measured by interferon, IFN, gamma ELI spot assays. 7. Significant levels of antibodies to VEV, but not E. This article was authored by Kali E. Bounds, Francis E. Terry, Leonard Moise, and others. We are article.tv, links in the description below.