 Stylization is a process of complete removal of microorganisms including their spores from an environment. Stylization is usually achieved by physical method, chemical method and by mechanical method. In this practical, we will demonstrate the physical method of Stylization. Physical method of Stylization is usually done by dry heat, moist heat and by radiation. First of all, dry heat. In dry heat, we will start from hot air oven. In hot air oven, a temperature of 160 degrees Celsius for 2 hours is sufficient for Stylization. Hot air oven is usually used to sterilize glassware, porcettes, spatulas and pipettes. Now, let's talk about its construction and operation details. Hot air oven comprises of a door, shelves, a thermostat and a circulating fan. Hot air oven is an insulated chamber in which electrically generated heat is confined. Circulating fan helps to distribute heat horribly into the chamber. Electrical coils for heat generation are located at the bottom of the hot air oven. The other method of physical Stylization using dry heat is flaming. Flaming involves destruction of microbes by burning. Flaming is used to sterilize in a plating loop, porcettes and spatulas. In flaming, take an article in the flame when an article in the flame turns red, Stylization is done. The physical method of Stylization is done by using moist heat. The laboratory apparatus that is designed using moist heat is AutoClave. The basic principle of AutoClave is to expose each and every article at 121 degrees Celsius at 15 psi for 15 minutes. AutoClave works by using heat to kill microbes and their spores. Heat is delivered by using pressurized steam. Pressurization allows the steam to reach at high temperature used for Stylization.