 Applications of PCR. There is a long list of applications of PCR. If we see with the help of polymerase chain reaction, we can detect the infectious diseases like TB, hepatitis B, C, and viral bacterial DNA, fungal infections. All these can be detected with the help of polymerase chain reaction. On the other hand, we can also diagnose latent viruses. PCR also have applications in the field of forensic DNA. So DNA fingerprinting is usually performed with the help of polymerase chain reaction. Other applications of the polymerase chain reaction are detection of the mutations in case of inherited disorders or the carriers of certain diseases can also be identified with the help of polymerase chain reaction. Prenatal diagnosis is also for certain diseases is also performed with the help of polymerase chain reaction. Detection of pathogens in case of prenatal diagnosis of the DNA, drug discovery, classification of the organism and their genotyping now also performed with the help of polymerase chain reaction. So polymerase chain reaction also have applications in the field of detection of pathogens, drug discovery, as well as classification of the organisms. Other applications of the polymerase chain reaction is in the field of molecular archaeology, molecular epidemiology, molecular ecology in case of bioinformatics, genomic, genome cloning, site directed mutagenesis, and gene expression studies are also performed with the help of polymerase chain reaction. Polymerase chain reaction also have applications in clinical microbiology, like identification of certain bacteria, slow growing bacteria, bacterial antibiotic resistance genes, and in case of viruses, almost all the viruses can be identified with the help of polymerase chain reaction, not only identification of the viruses, but also we can check the viral load with the help of real-time PCR. In case of clinical microbiology, polymerase chain reaction also has applications in further identification of different fungal species like aspergillus, candida, plasmodium, trypanosoma, leshmenia, and babesia species can also be identified by using the polymerase chain reaction. So we can say that polymerase chain reaction is the latest technique of the molecular biology or of biotechnology, which have a vast application in almost every sub-discipline of the biotechnology.