 Kytosan is a natural polymer derived from the deacetylation of chitin, which is an abundant carbohydrate found in the shells of various marine and terrestrial organisms. It has been widely used to construct nanoparticles, n-piece, which are biocompatible, biodegradable, non-toxic, easy to prepare, and can be used as effective drug delivery systems. Additionally, kytosan n-piece have been employed in gene and vaccine delivery, as well as advanced cancer therapies, and they can also serve as new therapeutic tools against viral infections. This review summarizes the latest advances in the use of kytosan-based n-piece as nucleic acid delivery vehicles and gene therapy vectors. Particular attention is paid to the technological aspects of kytosan complexes for nucleic acid delivery. This article was authored by Maria Cariani, Theodorsen Tokas, Athanasius Scandalis, and others. We are article.tv, links in the description below.