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2D Gel Electrophoresis (11) Mass Sample Preparation

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Published on May 16, 2011

( http://www.abnova.com ) - Mass Sample Preparation AbVideo focuses on the workflow of mass spectrometry before samples analysis. The entire process includes protein bands excision, organic solvent wash, in-gel digestion and peptide extraction. Two-dimensional gel electrophoresis, (2-DE or 2D electrophoresis), is a form of gel electrophoresis commonly used to isolate the proteins in a sample for further characterization by mass spectroscopy. Mixtures of proteins are separated in the 1st dimension by their charge (isoelectric focusing (IEF)). Then in the 2nd dimension, proteins are separated by their size (molecular weight). More videos at Abnova http://www.abnova.com

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