 Recent interest in mushrooms and their components as potential therapies for mental health, along with recent government and health authority approvals, has necessitated a more comprehensive understanding of their effects on the cellular microenvironment of the brain. Ammonite muscaria has been ingested as a treatment for a variety of ailments for centuries, most notably those affecting the central nervous system and conditions associated with neuro-inflammation. However, the effects of these extracts on neuro-inflammatory cells, such as microglia, are unknown. The effect of commercially sourced F muscaria extract AMEV1 on human microglial cell line, HMC3, expression of surface receptors such as CD86, CXCR4, CD45, CD125 and TLR4 was determined by flow cytometry. AMEV1 a pregulated expression of all of these receptors. The effect of AMEV1 on HMC3 production of IL-8 and IL-6 was determined and compared to tumor necrosis factor, TNF, polyanacinic polycytodilic acid, poly. This article was authored by Ashley Wagner, Marcus Pihar, Marcus Pihar, and others. We are article.tv, links in the description below.