 A novel functionalization method for gold nanorods, GNRs, intracellular delivery system was developed using electrostatic layer by layer assembly, in which multi-layer polyelectrolyte coated GNRs were used as vector and antisense oligodeoxynucleotides, acids, as therapeutic gene. The positively charged GNRs were modified with two successive polyelectrolyte layers to improve biocompatibility and facilitate further biofunctionalization. Characterized by UVvis spectroscopy, TM, and MTTSA, the multi-layered polyelectrolyte functionalized GNRs were used as vector for acids. Electrochemical impedance spectroscopy, EIS, was employed to confirm the formation of the GNRSS-ODNS conjugates through electrostatic interaction. The cellular uptake and delivery efficiency of the GNRSS-ODNS conjugates as well as cellular apoptosis induced by the acids transfected with gold nanorods were investigated using confocal microscopy and flow cytometry, exhibiting efficient intracellular delivery and improved anti-tumor activity of acids by the polyelectrolytes coated GNRs carriers. This article was authored by Shouhoi Chen, Yushuan Ji, Qiang Lian, and others. We are article.tv, links in the description below.