 This study investigated the effects of waterlogging at different growth stages on the photosynthesis enzyme activity, chlorophyll content, malandialdehyde, MDA, content, photosynthetic parameters, dry matter accumulation, and grain yield of two sorghum hybrid varieties, genuoliang-01, Jn-01, and genes R31, Jz-31. The study found that waterlogging had a negative effect on sorghum growth, with the greatest damage occurring when plants were waterlogged during their early developmental stages. This suggests that it is important to select appropriate genotypes and implement proper management strategies to reduce the risk of waterlogging-induced crop losses. This article was authored by Rui Deng Zhong, Zhong Xiaoyu, Xiaofei Chen, and others.