 A plasmin-enhanced fluorescence-based method for detecting low concentrations of Phase 3 Plus with improved sensitivity was developed using O1AG4 at CO2 nanoparticles dispersed in FITC solution, resulting in a quantized limit of detection towards Phase 3 Plus of 20 Nm compared to 4.6 Mu M for pure FITC solution. This article was authored by Lin Zhou, Han Zhang, Yan Pingwan, and others.