Table of Contents:
00:09 Lecture 3.5: Potentiometric Sensors Why are Biomolecules Charged?
02:24 Outline
03:02 Transistors and Biomolecules
03:54 Charge States of DNA controlled by pH
06:15 Average Charge per Base at pH=7
08:35 Average Charge per base at any pH
11:06 Outline
11:28 Biopolymers: Protein
13:06 Protein is composed of 20 Amino Acid
13:47 How to Calculate Protein Charge
15:22 Example: Prostate Specific Antigen
16:49 Isoelectric Points and Protein detection
17:27 Outline
17:49 Recall: Biomolecules and surface charges
19:03 Charges must be calculated self-consistently
20:53 Theory and Experiment: PSA
22:19 Biasing at the Point of Zero Charge
24:38 Conclusions
This video is part of the nanoHUB-U course "Principles of Electronic Nanobiosensors". (https://nanohub.org/courses/PEN)
This course provides an in-depth analysis of the origin of the extra-ordinary sensitivity, fundamental limits, and operating principles of modern nanobiosensors. The primary focus is the physics of biomolecule detection in terms of three elementary concepts: response time, sensitivity, and selectivity. And, it potentiometric, amperometric, and cantilever-based mass sensors to illustrate the application of these concepts to specific sensor technologies.
For more details see http://nanohub.org/u
