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Published on Feb 19, 2014
Table of Contents: 00:09 Lecture 3.5: Potentiometric Sensors Why are Biomolecules Charged? 02:24 Outline 03:02 Transistors and Biomolecules 03:54 Charge States of DNA controlled by pH 06:15 Average Charge per Base at pH=7 08:35 Average Charge per base at any pH 11:06 Outline 11:28 Biopolymers: Protein 13:06 Protein is composed of 20 Amino Acid 13:47 How to Calculate Protein Charge 15:22 Example: Prostate Specific Antigen 16:49 Isoelectric Points and Protein detection 17:27 Outline 17:49 Recall: Biomolecules and surface charges 19:03 Charges must be calculated self-consistently 20:53 Theory and Experiment: PSA 22:19 Biasing at the Point of Zero Charge 24:38 Conclusions
This course provides an in-depth analysis of the origin of the extra-ordinary sensitivity, fundamental limits, and operating principles of modern nanobiosensors. The primary focus is the physics of biomolecule detection in terms of three elementary concepts: response time, sensitivity, and selectivity. And, it potentiometric, amperometric, and cantilever-based mass sensors to illustrate the application of these concepts to specific sensor technologies.