 Open Comet is an open-source software tool designed to automatically analyze Comet assay images. It utilizes a novel approach to identify comets by analyzing their shape and intensity profiles, which is more accurate and efficient than manual methods. Additionally, its live analysis feature enables real-time analysis of Comet assays without having to wait for the results. This article was authored by Benjamin M. Jory, Jared Harvenka to Chulam, P. S. T. Adirajan, and others.