 A solution-based electrohydrodynamic EHD printing strategy was developed to create sub-microscale biopolymeric fibers that mimic native extracellular matrix ECM architecture which can enhance cellular performance. The process is applicable to biocompatible polycaprolactone PCL and produces water-stable sub-microscale fibrous architectures. These fibers exhibit unique capabilities in enhancing cellular adhesion, spreading, and orientation compared to conventional micro-scale fibers fabricated by melt-based EHD printing. The proposed solution-based EHD printing process provides a promising strategy for creating sub-microscale biopolymeric architectures that can be further functionalized with bioactive components for enhanced tissue regeneration. This article was authored by Bing Zhong, Zhen Kang He, Qi Lei Yi, and others.